Customized Synthesized Peptide


Customized Synthesized Peptide/Proteins
Peptide Synthesis Service

In the quantitative proteomics research, several MS-based methodologies for relative quantification have been introduced for comparison of different proteomes from collected biological samples. Meanwhile, MS-based methods for absolute quantification of specific proteins have been developed to accurately determine the protein concentrations. According to the guidelines for bioanalytical methods, the establishment and validation of accurate analytic proposals require standard compounds of high purity for calibrating and quality controls. Currently the dominant quantitative strategy is usually a combination of shotgun method and isotope dilution strategy. The targeted proteins in the complicated biological samples would release free peptide fragments induced by specific enzymatic cleavage, and the stable peptides with unique primary sequences in the digest mixture would be utilized as surrogates for corresponding parent proteins, so the small-molecular peptides can be quantified to estimate the protein concentration.

Scientist’s at Creative Proteomics specialized in the custom synthesis of synthetic peptides and peptide based molecules, providing a confidential and efficient service at competitive prices.

The peptide synthesis service we offer includes:

Modification List
Modification Fluorescence/DyeLabeling D-amino acids & etc
Acetylation (N-Terminal) Biotin (N-Terminal,Y/NAhx) D-Arg,D-Cys,D-Asp,D-Asn,D-Glu,D-Gln,D-Ser,D-His,D-Thr,D-Trp
D-Lys,D-Tyr,D-Orn,Orn,Abu,Aib, (D)1-Nal, (D)2-Pal
(D)4-Cl-Phe,Nva,Nle,Hse,Hcy,Pen,Mpa
Acetylation (Lys) Biotin/FITC (Lysinsequence) D-Ala,D-Leu,D-Met,D-Pro,D-Val,D-Phe,b-Ala, pGlu, Hyp
Formylation (N-Terminal) FITC/5-FAM (N-Terminal,Y/NAhx) D-Ile
Fatty acid (N-Terminal) Dansyl (N-Terminal,Y/NAhx) Gamma-Glu, beta-Asp
Myristicacid (N-Terminal) MCA (N-Terminal) Dinitrobenzoylation (Lys)
Palmytolyl (N-Terminal) HYNIC (N-Terminal)
Cys(Acm), Cys(tBu) DTPA (N-Terminal) Ser (octanoicacid)
Benzyloxycarbonylation (CBZ)
Amidation (C-Terminal)
p-Nitroanilide (pNA,C-Terminal)
AMC (C-Terminal)
Succinylation (Suc,N-Terminal)
Carbamidomethylated
Cyclic peptide Quenched fluorescent peptide Multiple Antigenic Peptide (MAP)
Disulfidebridge1st Abz/Tyr (3-NO2) Asymmetric 4 branches
Amidecyclic (Sidechain,end) DABCYL Asymmetric 8 branches
Glu (EDANS) Crude peptide with HPLC analysis, AAA need toadd extra cost
KLH
BSA, and etc
Hydrophobic sequence peptides Phosphorylation (Tyr,Ser,Thr) peptides Methylated peptides
Tyr,Ser,Thr
Stable isotope labelled peptides Difficult sequences/Long Peptide Synthesis Other Peptide
I,A,V,R,K,F,G,L, etc. N-Methylamino acid (Ala,Phe,Leu,Ile,Val,Gly,Met)
& More.
Synthetic methods used in Creative Proteomics:

Fmoc and Boc methodologies are both employed, using solid and solution phase reactions. Boc-chemistry solid phase peptide synthesis allows us to synthesise difficult sequences, and gives a greater flexibility in the synthesis of modified peptides. Fmoc chemistry is most suitable for simple peptides and sequences prone to oxidation.

Quality assurance files supplied after synthetic:

All peptides are supplied with COA, RP-HPLC and Mass Spec data. CHN analysis (available, will be online soon), amino acid analysis and N-terminal sequencing can be supplied at an extra cost. View example data: COA, RP-HPLC and Mass Spec.

What purity should I order?

• The most common MS grade purity requested is >95%;
• Antibody production >85% purity is often sufficient;
• NMR and Crystallography >98% is recommended;
• Crude peptides (>50%) can be used for screening large numbers of peptides.
• Any other purity requirement, PLEASE FEEL FREE TO CONTACT US!

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